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p1np (bone formation)  (Immunodiagnostic Systems)

 
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    Immunodiagnostic Systems p1np (bone formation)
    P1np (Bone Formation), supplied by Immunodiagnostic Systems, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/p1np+(bone+formation)/p1np/pm40663393-222-12-23
    Average 90 stars, based on 1 article reviews
    p1np (bone formation) - by Bioz Stars, 2026-10
    90/100 stars

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    other:

    Article Title: Inhibition of AhR improves cortical bone and skeletal muscle function via preservation of neuromuscular junctions.
    Article Snippet: 556 Bone remodeling activity was quantified in the serum via measurement of P1np (bone formation; 557 Imnmunodiagnostic Systems #AC-33F1) and Tracp5B (bone resorption; Immunodiagnostic Systems 558 #SB-TR103) following manufacturer’s protocols.

    Article Title: Multiscale analysis and functional validation of the cellular and genetic determinants of skeletal disease
    Article Snippet: Serum bone resorption (C-terminal telopeptides of type 1 collagen (CTX)) and bone formation (N-terminal propeptide of type I procollagen (P1NP)) were determined by ELISA at P70 (Immunodiagnostic Systems Ltd, Boldon, Tyne & Wear, UK).

    Article Title: A Model Study to Evaluate Osseointegration and Fracture Healing Following Open Reduction and Internal Fixation (ORIF) in Diabetic Lewis Rats.
    Article Snippet: The supernatant containing sera was used for evaluation of bone formation (N-terminal propeptide of type 1 procollagen (P1NP), IDS AC-33F1), and bone resorption (C-terminal telopeptide of type I collagen (CTX-1), IDS AC-06F1) using colorimetric ELISA assays (Immunodiagnostic Systems, Tyne andWear, UK).

    Article Title: Fructooligosaccharides act on the gut–bone axis to improve bone independent of Tregs and alter osteocytes in young adult C57BL/6 female mice
    Article Snippet: To determine systemic alterations in osteoblast and osteoclast activities induced by treatments, serum indicators of bone formation, N-terminal P1NP, and bone resorption, C-telopeptide of type I collagen (CTX-1) were assessed using commercially available EIA kits (Immunodiagnostic Systems, Inc., Fountain Hills, AZ).

    Article Title: A Model Study to Evaluate Osseointegration and Fracture Healing Following Open Reduction and Internal Fixation (ORIF) in Diabetic Lewis Rats
    Article Snippet: The supernatant containing sera was used for evaluation of bone formation (N-terminal propeptide of type 1 procollagen (P1NP), IDS AC-33F1), and bone resorption (C-terminal telopeptide of type I collagen (CTX-1), IDS AC-06F1) using colorimetric ELISA assays (Immunodiagnostic Systems, Tyne and Wear, UK).

    Article Title: Comparative study in estrogen-depleted mice identifies skeletal and osteocyte transcriptomic responses to abaloparatide and teriparatide.
    Article Snippet: Fasting serum levels of the bone formation marker P1NP and the bone resorption marker CTX were measured using commercial kits from Immunodiagnostic Systems.

    Activity Assay:

    Article Title: Inhibition of AhR improves cortical bone and skeletal muscle function via preservation of neuromuscular junctions
    Article Snippet: .. Bone remodeling activity was quantified in the serum via measurement of P1np (bone formation; Imnmunodiagnostic Systems, AC-33F1) and Tracp5B (bone resorption; Immunodiagnostic Systems, SB-TR103) following the manufacturer’s protocols. ..

    Marker:

    Article Title: Long-term excessive salt consumption alters villous and crypt morphology and the protein expression of uroguanylin, TRPV6 and PMCA1b in the rat small intestine.
    Article Snippet: .. Bone formation marker (N-terminal propeptide of type 1 procollagen; P1NP; cat. no. AC-33F1; Immunodiagnostic Systems, Bolden, UK), and resorption marker (C-terminal telopeptide of type 1 collagen; CTX-1; cat. no. AC-06F1; Immunodiagnostic Systems) were determined by commercial ELISA kits according to the manufacturer’s instruction. ..

    Enzyme-linked Immunosorbent Assay:

    Article Title: Long-term excessive salt consumption alters villous and crypt morphology and the protein expression of uroguanylin, TRPV6 and PMCA1b in the rat small intestine.
    Article Snippet: .. Bone formation marker (N-terminal propeptide of type 1 procollagen; P1NP; cat. no. AC-33F1; Immunodiagnostic Systems, Bolden, UK), and resorption marker (C-terminal telopeptide of type 1 collagen; CTX-1; cat. no. AC-06F1; Immunodiagnostic Systems) were determined by commercial ELISA kits according to the manufacturer’s instruction. ..



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    Upregulation of mammalian Sterile 20-like kinase 4 (MST4) expression in peripheral blood mononuclear cells of clinical osteoporosis patients (OP). (A) Quantitative real-time polymerase chain reaction (RT-qPCR) detection of MST4 mRNA levels in peripheral blood mononuclear cells of OP and healthy controls (Control) ( n = 6). (B, C) Western blot analysis of MST4 protein expression levels (B) and corresponding statistics (C) in OP and Control ( n = 6). (D) Bone mineral density (BMD) levels in OP and Control. (E, F) Expression levels of bone formation markers osteocalcin (OC) (E) and <t>procollagen</t> type I N -terminal propeptide <t>(P1NP)</t> (F) in OP and Control. (G, H) Expression levels of bone formation markers β-CrossLaps (β-CTX) and tartrate-resistant acid phosphatase 5b (TRAcP 5b) in OP and Control. (I) Receiver operating characteristic (ROC) curve analysis of MST4 expression for predicting fragility fractures in OP ( n = 60). ∗ P < 0.05 compared between groups, n = 60 per group（OP 60，Control 60); ns: not significant.
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    Effects of TFE treatment on (A) 17β-estradiol, (B) <t>P1NP,</t> and (C) CTX-I. Values are presented as mean (SD; Sham, n = 11; OVX, n = 10; TFE, n = 11). CTX-I, C-terminal telopeptides of type I collagen; P1NP, type 1 collagen amino propeptide; OVX, ovariectomy; Sham, sham-operation; TFE, total flavonoids of Epimedium; Ve, vehicle. TFE treatment inhibited bone turnover in OVX rats. Serum 17β-estradiol was significantly reduced while levels of CTX-I were higher in OVX rats than in Sham rats. * P <0.05 and ** P <0.001 by One-way ANOVA with Bonferroni-correction.
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    The changes in bone metabolism parameters and serological markers in rats after 12 weeks of JWYHD intragastric treatment are described. (a) Micro-CT was used to scan three-dimensional reconstruction images of bone trabeculae. (b) BMD. (c) BV/TV. (d) Tb.Th. (e) Tb.N. (f) Tb.Sp. (g) The translocation expression of BMP2, a key protein in the pathway, was detected by immunofluorescence. (h) The protein expression was measured by WB after culturing for 1 week. (i) BMP2 translocation percentage. (j) Weight gain of rats. (k) ELISA quantified the content of bone resorption marker β -CTX in serum. (l) ELISA quantified the content of the bone formation marker <t>P1NP</t> in serum. (m)-(q) The quantitative measurement of protein expression (BMP2, P-SMAD1/5/8, RUNX2, OCN, PPAR γ ). The data were represented by mean ± SD ( n = 3), P < 0.05 were considered as significant difference: ∗ VS. SHAM group, # VS. M group.
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    procollagen type 1 n-terminal propeptide (p1np, a bone formation marker)  (Immunodiagnostic Systems)
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    Immunodiagnostic Systems procollagen type 1 n-terminal propeptide (p1np, a bone formation marker)
    The changes in bone metabolism parameters and serological markers in rats after 12 weeks of JWYHD intragastric treatment are described. (a) Micro-CT was used to scan three-dimensional reconstruction images of bone trabeculae. (b) BMD. (c) BV/TV. (d) Tb.Th. (e) Tb.N. (f) Tb.Sp. (g) The translocation expression of BMP2, a key protein in the pathway, was detected by immunofluorescence. (h) The protein expression was measured by WB after culturing for 1 week. (i) BMP2 translocation percentage. (j) Weight gain of rats. (k) ELISA quantified the content of bone resorption marker β -CTX in serum. (l) ELISA quantified the content of the bone formation marker <t>P1NP</t> in serum. (m)-(q) The quantitative measurement of protein expression (BMP2, P-SMAD1/5/8, RUNX2, OCN, PPAR γ ). The data were represented by mean ± SD ( n = 3), P < 0.05 were considered as significant difference: ∗ VS. SHAM group, # VS. M group.
    Procollagen Type 1 N Terminal Propeptide (P1np, A Bone Formation Marker), supplied by Immunodiagnostic Systems, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/p1np+(bone+formation)/p1np/pm32920088-71-22-57
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    Image Search Results


    Upregulation of mammalian Sterile 20-like kinase 4 (MST4) expression in peripheral blood mononuclear cells of clinical osteoporosis patients (OP). (A) Quantitative real-time polymerase chain reaction (RT-qPCR) detection of MST4 mRNA levels in peripheral blood mononuclear cells of OP and healthy controls (Control) ( n = 6). (B, C) Western blot analysis of MST4 protein expression levels (B) and corresponding statistics (C) in OP and Control ( n = 6). (D) Bone mineral density (BMD) levels in OP and Control. (E, F) Expression levels of bone formation markers osteocalcin (OC) (E) and procollagen type I N -terminal propeptide (P1NP) (F) in OP and Control. (G, H) Expression levels of bone formation markers β-CrossLaps (β-CTX) and tartrate-resistant acid phosphatase 5b (TRAcP 5b) in OP and Control. (I) Receiver operating characteristic (ROC) curve analysis of MST4 expression for predicting fragility fractures in OP ( n = 60). ∗ P < 0.05 compared between groups, n = 60 per group（OP 60，Control 60); ns: not significant.

    Journal: Journal of Pharmaceutical Analysis

    Article Title: MST4 as a key driver of osteoclast activation in osteoporosis

    doi: 10.1016/j.jpha.2025.101401

    Figure Lengend Snippet: Upregulation of mammalian Sterile 20-like kinase 4 (MST4) expression in peripheral blood mononuclear cells of clinical osteoporosis patients (OP). (A) Quantitative real-time polymerase chain reaction (RT-qPCR) detection of MST4 mRNA levels in peripheral blood mononuclear cells of OP and healthy controls (Control) ( n = 6). (B, C) Western blot analysis of MST4 protein expression levels (B) and corresponding statistics (C) in OP and Control ( n = 6). (D) Bone mineral density (BMD) levels in OP and Control. (E, F) Expression levels of bone formation markers osteocalcin (OC) (E) and procollagen type I N -terminal propeptide (P1NP) (F) in OP and Control. (G, H) Expression levels of bone formation markers β-CrossLaps (β-CTX) and tartrate-resistant acid phosphatase 5b (TRAcP 5b) in OP and Control. (I) Receiver operating characteristic (ROC) curve analysis of MST4 expression for predicting fragility fractures in OP ( n = 60). ∗ P < 0.05 compared between groups, n = 60 per group（OP 60，Control 60); ns: not significant.

    Article Snippet: Bone metabolism markers in peripheral blood, such as the bone formation marker procollagen type I N -terminal propeptide (P1NP) (D711084-0048; Sangon Biotech Co., Ltd., Shanghai, China), osteocalcin (OC) (D711187-0048; Sangon Biotech Co., Ltd., Shanghai, China), and bone resorption markers like β-CrossLaps (β-CTX) (D711348; Sangon Biotech Co., Ltd., Shanghai, China), and tartrate-resistant acid phosphatase 5b (TRAcP 5b) (P0332; Beyotime Biotechnology, Shanghai, China), were measured using enzyme-linked immunosorbent assay (ELISA).

    Techniques: Sterility, Expressing, Real-time Polymerase Chain Reaction, Quantitative RT-PCR, Control, Western Blot

    Effects of TFE treatment on (A) 17β-estradiol, (B) P1NP, and (C) CTX-I. Values are presented as mean (SD; Sham, n = 11; OVX, n = 10; TFE, n = 11). CTX-I, C-terminal telopeptides of type I collagen; P1NP, type 1 collagen amino propeptide; OVX, ovariectomy; Sham, sham-operation; TFE, total flavonoids of Epimedium; Ve, vehicle. TFE treatment inhibited bone turnover in OVX rats. Serum 17β-estradiol was significantly reduced while levels of CTX-I were higher in OVX rats than in Sham rats. * P <0.05 and ** P <0.001 by One-way ANOVA with Bonferroni-correction.

    Journal: Frontiers in Endocrinology

    Article Title: Effects of Total Flavonoids of Epimedium on Bone Marrow Adipose Tissue in Ovariectomized Rats

    doi: 10.3389/fendo.2022.900816

    Figure Lengend Snippet: Effects of TFE treatment on (A) 17β-estradiol, (B) P1NP, and (C) CTX-I. Values are presented as mean (SD; Sham, n = 11; OVX, n = 10; TFE, n = 11). CTX-I, C-terminal telopeptides of type I collagen; P1NP, type 1 collagen amino propeptide; OVX, ovariectomy; Sham, sham-operation; TFE, total flavonoids of Epimedium; Ve, vehicle. TFE treatment inhibited bone turnover in OVX rats. Serum 17β-estradiol was significantly reduced while levels of CTX-I were higher in OVX rats than in Sham rats. * P <0.05 and ** P <0.001 by One-way ANOVA with Bonferroni-correction.

    Article Snippet: Bone turnover biomarkers included bone formation marker type 1 collagen amino propeptide (P1NP) and bone resorption marker C-terminal telopeptides of type I collagen (CTX-I) (Immunodiagnostic Systems Nordic A/S, Denmark).

    Techniques:

    The changes in bone metabolism parameters and serological markers in rats after 12 weeks of JWYHD intragastric treatment are described. (a) Micro-CT was used to scan three-dimensional reconstruction images of bone trabeculae. (b) BMD. (c) BV/TV. (d) Tb.Th. (e) Tb.N. (f) Tb.Sp. (g) The translocation expression of BMP2, a key protein in the pathway, was detected by immunofluorescence. (h) The protein expression was measured by WB after culturing for 1 week. (i) BMP2 translocation percentage. (j) Weight gain of rats. (k) ELISA quantified the content of bone resorption marker β -CTX in serum. (l) ELISA quantified the content of the bone formation marker P1NP in serum. (m)-(q) The quantitative measurement of protein expression (BMP2, P-SMAD1/5/8, RUNX2, OCN, PPAR γ ). The data were represented by mean ± SD ( n = 3), P < 0.05 were considered as significant difference: ∗ VS. SHAM group, # VS. M group.

    Journal: Evidence-based Complementary and Alternative Medicine : eCAM

    Article Title: Jiawei Yanghe Decoction Regulates Bone-Lipid Balance through the BMP-SMAD Signaling Pathway to Promote Osteogenic Differentiation of Bone Mesenchymal Stem Cells

    doi: 10.1155/2022/2885419

    Figure Lengend Snippet: The changes in bone metabolism parameters and serological markers in rats after 12 weeks of JWYHD intragastric treatment are described. (a) Micro-CT was used to scan three-dimensional reconstruction images of bone trabeculae. (b) BMD. (c) BV/TV. (d) Tb.Th. (e) Tb.N. (f) Tb.Sp. (g) The translocation expression of BMP2, a key protein in the pathway, was detected by immunofluorescence. (h) The protein expression was measured by WB after culturing for 1 week. (i) BMP2 translocation percentage. (j) Weight gain of rats. (k) ELISA quantified the content of bone resorption marker β -CTX in serum. (l) ELISA quantified the content of the bone formation marker P1NP in serum. (m)-(q) The quantitative measurement of protein expression (BMP2, P-SMAD1/5/8, RUNX2, OCN, PPAR γ ). The data were represented by mean ± SD ( n = 3), P < 0.05 were considered as significant difference: ∗ VS. SHAM group, # VS. M group.

    Article Snippet: The corresponding ELISA kit was used to detect the levels of bone formation biochemical markers P1NP (Elabscience, China, #E-EL-R1414c) and bone resorption biochemical markers β -CTX (Elabscience, China, #E-EL-R1405c) according to the instructions of the manufacturer.

    Techniques: Micro-CT, Translocation Assay, Expressing, Immunofluorescence, Enzyme-linked Immunosorbent Assay, Marker